In Amnio MRI of Mouse Embryos

In Amnio MRI of Mouse Embryos

Mouse embryo imaging is conventionally carried out on ex vivo embryos excised from the amniotic sac, omitting vital structures and abnormalities external to the body. Here, we present an in amnio MR imaging methodology in which the mouse embryo is retained in the amniotic sac and demonstrate how important embryonic structures can be visualised in 3D with high spatial resolution (100 µm/px). To ...

Diffusion MRI of in utero mouse embryos

Introduction The mouse embryo is a widespread animal model for human development disorders. MRI offers a unique, noninvasive approach for imaging normal and abnormal mouse embryo development in later gestational stages. One of the difficulties of imaging in utero mouse embryos is the lack of tissue contrast exhibited by the embryo at high magnet field strengths. This project explores the use of...

High-resolution MRI of early-stage mouse embryos.

Both the availability of methods to manipulate genes and the completion of the mouse genome sequence have led to the generation of thousands of genetically modified mouse lines that provide a new platform for the study of mammalian development and developmental diseases. Phenotyping of mouse embryos has traditionally been performed on fixed embryos by the use of ex vivo histological, optical an...

CIGARETTE SMOKE/ETHANOL-INDUCED LIMB DEFECTS IN MOUSE EMBRYOS

We have produced a spectrum of limb defects in developing mouse embryos by exposing the mother to smoke from cigarettes with different nicotine concentrations, ethanol, and a combination of ethanol and cigarette smoke. The critical time of exposure was determined to be during day 10 of gestation (vaginal plug=day 0). This time is prior to the critical events which occur between the apical e...

TERATOGENIC EFFECTS OF LITHIUM CARBONATE ON DEVELOPMENT OF MOUSE EMBRYOS